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The Web KSU Aquaculture

DIVISION
OF
AQUACULTURE

Baitfish
Bass, Hybrid
Bass, Largemouth
Bluegill, Hybrid
Carp, Common
Catfish
Crappie
Paddlefish
Perch, Yellow
Prawn (Shrimp)
Red Claw Crayfish
Sturgeon
Tilapia
Trout
Walleye
Contact
Nathan Cochran
with Web site
comments.

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Research on floating Raceways for Nursery
Production of Largemouth Bass (Phase I)

Sandhya Lamichhane’s research will document food availability, and selectivity of phase I largemouth bass in nursery ponds and raceways with a managed bloom. A bloom in ponds refers to an excessive growth of algae or other microscopic organisms, such as phytoplankton, that can cause the water to appear green, brown, or red. These blooms are often visible on the surface as a dense layer or scum. Sandhya will compare zooplankton abundance in managed nursery ponds and stomach contents of phase I LMB in both ponds and floating raceways.


Blooms are important as they can have both positive and negative impacts on the ecosystem. On one hand, they play a vital role in the food chain as a primary food source for various aquatic organisms and help maintain the overall biodiversity of the pond. Additionally, they contribute to oxygen production through photosynthesis.

However, excessive blooms can also have negative consequences. When the bloom is too dense, it can block sunlight from reaching deeper layers of water, impacting the growth of underwater plants and disrupting the habitat for fish and other organisms. As these blooms eventually die off, decomposition by bacteria can deplete oxygen levels in the water, leading to a phenomenon known as hypoxia or dead zones, which can be harmful
to aquatic life.

Andrew Stephan’s will compare open pond culture and a floating raceway system for nursery production of phase I largemouth bass in ponds with managed blooms. A secondary task Andrew will compare these results with floating raceways in a naturally fertile (fed) pond with an unmanaged bloom.

Photos by Charles Weibel

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Dr. Andrew Ray Presents at Shrimp Summit

Dr. Ray traveled to the inaugural Florida Shrimp Summit in Fort Pierce, Florida. He gave a presentation titled "Indoor Shrimp Farming Systems in the U.S." which covered several different strategies and technologies that can be used to successfully grow shrimp indoors. Some of the information was gathered from a comprehensive survey of U.S. shrimp farms that Dr. Ray conducted, other information he had gathered by visiting farms across the world and conducting state- of-the-art research right here at KSU. There were about 50 people in the audience and they had a very positive reaction to Dr. Ray's presentation. This is another example of the many ways that KSU Aquaculture is reaching a broad set of constituents to deliver impactful information that helps drive the aquaculture industry forward.

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Sagun Samples Flounder Research Project

Ms. Sagun Chhetri conducted a sample of her research project with the assistance of Dr. Ray and his crew, focusing on the growth of flounder under varying stocking densities. The results may indicate that flounder exhibit superior growth in low stocking density conditions, with a current average weight exceeding 300 grams, followed by medium and high densities. Overall, the survival rate has been satisfactory, with minimal instances of mortalities observed thus far. The projected completion date for Ms. Chhetri's research is approximately in the middle of June 2024. At harvest, Sagun will obtain her last sample to evaluate fish growth and extract blood samples for analysis. Regarding the extraction of DNA from fin clips, the objective is to examine whether genes associated with stress exhibit greater expression in fish reared under high stocking density compared to those in low density tanks.

It is important to note that the specific genes and stress-related pathways in fish can vary depending on the species and the particular stressor applied. For example, Cortisol, a hormone involved in the fish stress response, plays a significant role. Genes responsible for cortisol synthesis, such as the steroidogenic acute regulatory protein (StAR), cytochrome P450 11B (CYP11B), and 11-beta-hydroxylase (CYP11B1), are associated with the fish's ability to regulate stress. Furthermore, it is crucial to consider that the expression of these genes can be influenced by factors such as the specific stressor, duration and intensity of the stress, as well as inherent variations among fish species. Researchers conduct comprehensive studies to identify and comprehend the specific genes and pathways implicated in stress responses among different fish species, aiming to gain insights into their adaptive capabilities and responses to environmental stressors.

Photos by Charles Weibel

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Aquaculture Students Bring Home Awards From
Aquaculture America Conference

Kentucky State University is proud to announce the outstanding achievements of its aquaculture graduate students at the prestigious Aquaculture America conference and exposition, held in San Antonio, Texas in February 2024. This highly regarded national event provides a platform for students to showcase their research projects and compete among peers and experts in the field. With the opportunity to submit abstracts for consideration, Kentucky State University sent a group of exceptional students to participate in oral and poster presentations at this esteemed gathering. This experience enabled them to demonstrate their knowledge in the STEM areas and aquaculture, while answering insightful questions from industry professionals and experts. The conference not only provided the students with a chance to present their research in a professional setting but also exposed them to cutting-edge aquaculture research and industry advancements shared by esteemed professionals. Additionally, the event fostered valuable networking opportunities among like-minded peers and industry leaders.

We are thrilled to share with you the impressive accomplishments of our students, who truly rose to the occasion and brought home remarkable accolades for Kentucky State University. Noah Jenkins secured a commendable Second Place for his outstanding oral presentation, while Faith Ohwofasa dazzled the judges with a striking second- place win for her poster presentation. Ashton Schardt's remarkable oral presentation earned him a well- deserved third-place recognition. Their hard work and dedication have paid off, and their achievements will be published in the esteemed Aquaculture Magazine. These triumphs not only reflect the exceptional talent and commitment of our students but also bring great recognition to Kentucky State University's Aquaculture Program, College of Agriculture, and our institution as a whole.

We are immensely proud of their accomplishments and their representation of our university on a national stage. We extend our sincere congratulations to Noah Jenkins, Faith Ohwofasa, Ashton Schardt, and all the students who participated in the conference. Their success underscores the exceptional educational opportunities and support provided by Kentucky State University.

Photos by Charles Weibel

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Dole Daleiden Defends His Thesis

We are thrilled to share the exciting news of Cole's successful thesis defense at the Kentucky State University Aquaculture Research Center! With his presentation titled "Evaluation of Productivity Traits, Growth, and Sex Proportion in Three Strains of Nile Tilapia," Cole showcased his expertise and dedication to his research. The atmosphere during the presentation was delightful, with pizza, an ice cream bar, and other tasty snacks provided to keep the audience satisfied and engaged. Despite the demands of a long Friday afternoon, Cole's hard work and preparation paid off, making him the newest graduate student to successfully defend his thesis. We extend our warmest congratulations to Cole on this significant milestone in his academic journey. None of this would have been possible without the guidance and support of his advising professor, Dr. Noel Novelo, who played a crucial role in shaping Cole's research and guiding him toward success. Cole's achievement is a testament to his commitment, perseverance, and intellectual prowess. Once again, congratulations, Cole! Your accomplishment is well-deserved, and we wish you continued success in all your future endeavors.

Photo by Charles Weibel

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Ashton Schardt Selected as Graduate Student of the Year

We are delighted to announce that Ashton Schardt has been selected as the recipient of the 2024 Outstanding Graduate Student of the Year Award. This prestigious accolade is determined annually by the Aquaculture Faculty, with input from various factors such as academics and leadership. Under the guidance and mentorship of Dr. Rossi from the Aquaculture Research Center Nutrition Lab, Ashton has displayed exemplary dedication and commitment to his studies. His impressive academic achievements and passion for the field of aquaculture have set him apart as a deserving candidate for this recognition. Furthermore, Ashton's active involvement in the AQUACULTURE AMERICA Student Sub Unit and his participation in other student activities have showcased his exemplary leadership skills and commitment to contributing to the academic community. His thoughtful attention to detail and diligent work ethic have greatly enriched the programs and initiatives at KSU and the Aquaculture Research Center. We extend our warmest congratulations to Ashton Schardt for this well-deserved honor and commend him for his outstanding contributions to the field of aquaculture.

Photo by Charles Weibel

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Dr. Ray Visit Largest Indoor Shrimp Farm

Dr. Andrew Ray recently had the opportunity to visit the largest indoor shrimp farm in Europe to evaluate their technology and provide recommendations for potential improvements. Located near Madrid, Spain, this farm has been operating for over 15 years, is currently undergoing expansion, and relies heavily on solar energy for power. While pictures from inside the farm cannot be shared due to trade secrets, the farm constantly strives to enhance sustainability, product quality, and production levels through continuous innovation. This collaboration allows Dr. Ray to contribute to the growth of the global industry while benefiting from the groundbreaking research conducted at Kentucky State University.

Europe ranks as the third-largest market for Pacific white shrimp worldwide, following the United States and China. In 2019, Europe imported an estimated EUR 1.9 billion worth of Pacific white shrimp. The volume of Pacific white shrimp imports in Europe for 2019 reached approximately 370,000 tonnes, a 7.5% decrease compared to 2018. Key European importers include France, Spain, the United Kingdom, Italy, the Netherlands, Germany, and Belgium, which collectively account for around 80% of the total European Pacific white shrimp imports.

In the United States, shrimp consumption has been steadily increasing since 2013, reaching a peak of 5.9 pounds per person in 2021. As of 2023, the US shrimp market size has reached 953,200 tons. Shrimp consumption represents 38% of the annual seafood consumption in the US, surpassing combined consumption of canned tuna, tilapia, Alaska pollock, pangasius, cod, and crab.

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Three Kentucky State University Aquaculture Students Graduate Earn Their Masters
in Aquaculture and Aquatic Sciences

We are delighted to share the remarkable achievements of three outstanding aquaculture graduate students from Kentucky State University. Uchechukwu Ohajiudu, Gaynu Rana, and Aakriti Khanal proudly received their well-deserved diplomas at the 2024 Kentucky State University Commencement ceremony.

Having successfully completed all course requirements and other necessary qualifications, Uchechukwu,
Gaynu, and Aakriti made a conscious decision to return to their alma mater to personally accept their diplomas. This symbolic gesture speaks volumes about their deep sense of pride and connection to
Kentucky State University. With their bright futures ahead, Uchechukwu and Aakriti have chosen to continue their educational pursuits by embarking on Ph.D. programs, further expanding their knowledge and expertise in the field of aquaculture. Meanwhile, Gaynu is currently engaged in searching for employment, aiming to contribute to the industry with the skills acquired during her studies. As we celebrate the accomplishments of these remarkable individuals, we are reminded of the profound impact that graduate students have on society. They are comparable to stars that have completed their constellations, illuminating the sky with the knowledge and wisdom gained from their dedicated efforts. Their unwavering determination, resilience, and relentless pursuit of excellence serve as an inspiration for future generations and leave an enduring legacy.

We extend our heartfelt congratulations not only to Uchechukwu, Gaynu, and Aakriti but also to all the remarkable graduates of 2024. Their achievements are a testament to their hard work, dedication, and commitment to personal and academic growth.

Photos by Charles Weibel

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The USAS Aquaculture Subunit Volunteer at the Legislator Fish Fry

The Aquaculture Subunit volunteers made a valuable contribution at the Legislator Fish Fry. Not only did Aquaculture students assist in preparing and serving food, but they also had the chance to network with others. Moreover, they proudly showcased their research posters, highlighting their important work. To top it all off, they enjoyed a delightful fish dinner, creating a memorable experience. We deeply appreciate their support in making this annual event a success.

Photos by Charles Weibel

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Sagun Stocks Olive Flounder into Recirculating Aquaculture Systems

The olive flounder (Paralichthys olivaceus) is a flatfish species that has been practically domesticated in Asia and is an excellent candidate for production in recirculating aquaculture systems (RAS). The fish has a very high market price, the meat is sashimi-grade, it grows quickly, and has a low feed conversion rate (FCR). To help optimize the production of this species in RAS, Sagun is conducting an experiment to evaluate the effects of stocking density on production metrics and stress-indicating compounds.

The Ray Lab has just finished constructing a state-of-the-art RAS which includes nine 1.2 m2 tanks.
Sagun and the team received approximately 2,000 fish from the University of Miami and raised them in nursery tanks for about three months. They then stocked the new tanks at three different commercial-level densities: 2.7, 5.4, and 8.1 kg/m2 to make the three treatments which include three replicate tanks each. Sagun will be measuring growth, biomass, survival, FCR, as well as comparative levels of cortisol, glucose, growth hormone, and insulin-like growth factor-1 in the fish blood. This work will be used to help determine the suitability of olive flounder in RAS so that domestic farmers may have new and valuable fish species to help expand U.S.
aquaculture.

Photos by Charles Weibel

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Ray Lab New Publication

The Ray Lab published a new manuscript in the prestigious journal Aquaculture. The paper is the result of Shrijan Bajracharya’s thesis research here at KSU with marine shrimp. It details an experiment that examined the effects of an in-house made sea salt compared to a commercial salt, the effects of clear-water culture systems versus more turbid systems, and the addition of sugar as a bacterial carbon source versus no extra carbon addition.

The results are somewhat nuanced, but in general, indicate that the combination of more turbid systems (with less filtration), no sugar addition, and the in-house made salt mix (which is half the cost of commercial salt) had the best results. Indications of success include improved animal growth rates, and optimal water quality in that treatment. These results are currently being used to help bring down production costs in the growing U.S. inland marine shrimp farming industry. There are currently six marine shrimp farms in Kentucky alone, each of which has expanded production, or intends to soon, to keep up with demand for high quality, fresh,
sustainably grown shrimp.

Click Here For Full Journal Article

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Proud Moment for Kentucky State University!
Our incredible faculty, staff, and students recently had the opportunity to shine at
Aquaculture America.

The third week of February, in sunny San Antonio, TX, our team left a lasting impression with their exceptional oral and poster presentations. The dedication and hard work of our students, faculty, and staff truly made our university stand out! Not only did our undergraduate and graduate students gain valuable knowledge from the event, but they also had a blast networking and attending socials with like-minded individuals. Making connections and forging new friendships in the aquaculture field is what it's all about!

Let's not forget the massive Aquaculture Trade Fair, boasting hundreds of booths showcasing the latest and greatest in the aquaculture industry. Our team had the chance to explore these exhibits, immersing themselves in the cutting-edge advancements of this rapidly evolving field.

What an incredible opportunity it was for our students to witness a wide range of presentations at Aquaculture America! From experts in the industry to students from various universities, this event provided valuable insight into the latest research and advancements in the field of aquaculture. Our students had the privilege to observe and learn from the best, gaining inspiration and expanding their knowledge base.

By witnessing the diverse research conducted by their peers, our students were exposed to different perspectives and innovative ideas. This exposure will undoubtedly contribute to their own growth as they continue their studies and research in the exciting world of aquaculture. Kentucky State University should take pride in the accomplishments of our faculty, staff, and students. They are the driving force behind our success, and their representation at Aquaculture America did us proud. Congratulations to everyone involved!

Photos by Charles Weibel

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What a Successful Career Fair it was, Thanks to the Incredible Support of the USAS Student Subunit

Kentucky State University graduating seniors had access to valuable information about our programs and career opportunities. The Student USAS Subchapter played a pivotal role in guiding these seniors towards a bright future.

During the event, we had the pleasure of engaging in one-on-one conversations with approximately 10-15 students. Their interest in our programs was genuine, and they discovered new possibilities here at KSU.
Interestingly, not a single one of these students had even heard of our aquaculture programs before the Career Fair. They were pleasantly shocked to realize that such incredible opportunities were right here on campus. This realization highlights the importance of introducing our programs to incoming freshmen. We must bridge this information gap and ensure that every student is aware of the fantastic academic journey that awaits them at Kentucky State University’s Land Grant Program and the Aquaculture Research Center. As we move forward, it is imperative to focus on spreading the word about our exceptional programs, ensuring that every new freshman is informed and empowered to make the most of their time at KSU.

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Tates Creek Middle School Visits the Kentucky State University Aquaculture Research Center

On February 29, 2024, Seventeen bright students from Mr. Eli Parham's Agriculture class explored the incredible world of aquaculture. During their tour Mr. Parham's teaching philosophy emphasizes hands-on and project-based learning, creating an environment where students can thrive and gain
practical skills.

Tates Creek Middle School students have already delved into various agricultural disciplines, such as hydroponics, plant science, food science, and seed-to-plant experiences. Their enthusiasm for learning was evident as they soaked up knowledge during their visit to the Aquaculture Research
Center.

Exciting news! Tates Creek Middle School is currently in the planning stages of constructing an outdoor classroom/greenhouse, and they've expressed their interest in partnering with Kentucky State University for this endeavor. This collaborative effort holds immense potential for further nurturing the passion for agriculture among these young minds.

During the tour, our team had an absolute blast interacting with the students, who not only asked wonderful questions but also got a little wet while feeding the hungry tilapia. Their feedback through surveys confirmed their enthusiasm for the research projects they discovered, including aquaponics and the intriguing Olive Flounder.

KSU Aquaculture Extension and Research Associate, Chelsea Walling is thrilled to continue working closely with Mr. Parham and his dedicated students, fostering a fruitful collaboration in the field of agriculture. Together, we can cultivate knowledge, inspire innovation, and make a lasting impact on the future of aquaculture!

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"Expanding Horizons: Flounder Finds a Home at Kentucky State University"

Kentucky State University’s Aquaculture Research Center is embarking on an exciting venture by introducing a new saltwater species of fish, Flounder, into their aquaculture research efforts. There are several species of flounder that are commonly raised in aquaculture. Some of the main species include Summer Flounder (Paralichthys dentatus), Winter Flounder (Pseudopleuronectes americanus), Southern Flounder (Paralichthys lethostigma), and Olive Flounder (Paralichthys olivaceus). KSU will be conducting research on the Olive Flounder.

The Olive Flounder is a highly prized species in aquaculture, particularly in East Asia. It is known for its delicious taste and firm, white flesh. Olive flounder is commercially cultivated in countries like Japan and South Korea and holds significant economic value in the seafood market. Over the past months, the dedicated team led by Ray and graduate student Sagun Chhetri have been diligently working to nurture and grow flounder, aiming to reach a size of 30 grams or more before transferring them to a state-of-the-art Recirculating Aquaculture System (RAS). This elaborate RAS will provide an optimal environment for the flounder to thrive and be studied extensively. The team's efforts and expertise in fish cultivation will culminate in the commencement of the first flounder research study, which is projected to kick off in late February.

This groundbreaking initiative demonstrates KSU's commitment to pushing the boundaries of aquaculture research. By venturing into the cultivation and research of flounder, a valuable saltwater species, the university aims to contribute valuable insights to the field of aquaculture that can drive innovation and sustainability.

Photos by Charles Weibel

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Third Thursday Thing: Aquaculture

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Faith Ohwofasa Harvested Her Largemouth Bass Research Project

IMPACT OF LOW WATER TEMPERATURE ON GROWTH, FEED
CONSUMPTION, AND FEED EFFICIENCY OF JUVENILE
LARGEMOUTH BASS Micropterus nigricans

Faith Ohwofasa harvested her Largemouth Bass (LMB) research project during the holiday break. Growth of LMB as a food fish in Kentucky is constrained by a modest growing season. Her study examines growth, feed consumption, and feed efficiency of LMB at temperatures occurring in the spring or fall in temperate regions. Data generated will be instrumental in developing a practical feeding protocol for Largemouth Bass to
take advantage of growth potential throughout the year. Miss Ohwofasa’s study was conducted over a 9-week period at the Kentucky State University’s Aquaculture Research Center. Seven individual recirculating systems with each system composed of four replicate aquarium tanks were randomly assigned a temperature treatment (9, 12, 15, 18, 21, 24, and 27?).

Prior to stocking the experiment, individual (LMB) were injected with a Passive Integrated Transponder (PIT tag). Each tank was stocked with ten feed-trained juvenile LMB (56.2 ± 0.8 g) and fed once daily by hand to apparent satiation with slow-sinking 6.5 mm pellet of commercial trout feed at (45% protein, 20% fat). Water quality was monitored to maintain conditions suitable for growth. The weight, length, and tag number for each fish was recorded at the end of the research project.

Significant differences will be determined with a One-Way ANOVA, and the relationships described with a regression analysis. The ongoing analysis of weekly feed consumption measurement reveals an excellent fit between feed intake and water temperature. Full results from the study will be presented at a later date.

Photo by Charles Weibel

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Ashton Schardt Completes His Mussel Nutrition Research Project

Freshwater mussel nutrition research is in the infancy of discovering how certain commercial products provide essential nutrients for freshwater mussels. We still do not know exactly what nutrients they hold on to and which ones they send downstream. Commercial marine algae feeds and additives have been used in captive freshwater mussel culture as an alternative to live algae.

Ashton’s thesis assesses various commercial algae feeds and additive treatments. Nine experimental treatments were formulated with a negative control, four treatments of paste algae, and four treatments of freeze-dried algae with additives (probiotics, worm casing, and a commercial algae replacement).

Twenty mussels per tank are stocked into 36, 9L tanks in a static system. The mussels are fed daily after the 30% water exchange from each tank. Water exchanges happen every 8 hours; over 24 hours the tanks receive a 100% water exchange. The mussels will be sampled every two weeks to assess length, growth, and mortalities. Pictures are taken to be used for growth analysis. Growth is measured using a computer program called Image-Pro Plus, an imaging software that measures the animals in millimeters. The feed is adjusted after each sampling event according to weight gain.

Following the conclusion of the trial, results including final weight, biweekly growth rate, bi- weekly length measurements, and survival will be presented. Further analysis will be conducted for protein, energy, potential amino acid, fatty acid, and carbohydrate profiles in the mussels.

Following the conclusion of the trial, results including final weight, biweekly growth rate, bi- weekly length measurements, and survival will be presented. Further analysis will be conducted for protein, energy, potential amino acid, fatty acid and carbohydrate profiles in the mussels.

Photos by Charles Weibel

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Cole Daleiden Harvest Thesis Research Project

November marked the successful completion of a 90-day comparative growout study designed to evaluate three strains of Nile Tilapia produced by two methods of sex control. One strain of genetically male tilapia was produced at Kentucky State University using YY males and XX females. The other two strains were commercially available and produced using hormonal sex reversal. Genetically male tilapia and hormonally sex-reversed tilapia are both methods of sex control to increase male proportion. For producers, all- male populations are limited to those interested in raising fish without synthetic hormone usage. Nile Tilapia research at KSU addresses these concerns by evaluating genetically male tilapia as an alternative to synthetic hormones for all-male production.

The final sampling event was accomplished in excellent time thanks to the contribution of many students and staff members of the Aquaculture Research Center. There were four stations working simultaneously, each station with its own team and responsibilities. Stations 1 and 2 assessed nearly 600 fish in total, recording size measurements, tank populations, and euthanizing a set number of fish. Team three evaluated all the euthanized fish for additional size and morphometric measurements. Team 4 was responsible for final dissections to identify the sex and processed weight of each euthanized fish. The teams worked in coordination with each other, and every volunteer did an outstanding job completing the various duties and ensuring the smooth completion of this event.

Photos by Charles Weibel

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Electro-Fishing Field Trip

Kentucky State University’s Aquaculture Graduate Students launched two boats into the Kentucky River this week to go electro-fishing as part of their Fish Physiology and Morphology class. Dr. Semmons and a two-man crew were in the lead boat, the shocking boat. Dawson followed the lead boat with a crew of 4 to 5 people to net up any fish that Dr. Semmens's crew missed.

After catching several fish, Dawson’s boat would return to the boat ramp to unload the captured fish. The crew at the boat ramp would document fish identification, lengths, and weight of each fish caught.

Each student would pick a fish species to dissect and prepare a presentation on at a later date.

This field trip allowed students hands-on experience with fish population surveys and boat safety.

Electroshocking, also known as electrofishing, is a common scientific technique used in fisheries management and research to survey fish populations in lakes, rivers, and streams.

Overall, electroshocking is a valuable tool in fisheries management and research as it provides valuable data about fish populations, including abundance, distribution, and size structure. This information helps professionals make informed decisions on conservation efforts, stocking programs, and habitat management to ensure the sustainable management of fish populations and ecosystems.

The students were lucky enough to catch several species of fish, including, buffalo, red horse, silver and blue sucker, freshwater drum, gar, Spotted bass, largemouth bass, smallmouth bass, gizzard shad, bluegill, channel catfish, and more.

Photos by Charles Weibel

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Faith Ohwofasa Conducts Largemouth bass Research

Largemouth bass were stocked into aquaria in the Hatchery on 10/18/23. This experiment supports work to devise a protocol for feeding largemouth bass in ponds and floating raceways at any time of the year.

An important goal among KY bass growers is to reach an average weight of 1.25 lb (545 g) by the end of the second season. First year growth is exceedingly important, but the season is short for fingerlings produced in KY. It is useful to obtain growth even as water temperature declines in the fall and through the winter. Previous work by the team focused on growth and feed consumption of fingerling and juvenile LMB at temperatures between 18 and 33° C.

This experiment will generate data from pit- tagged juvenile LMB in RAS systems maintained at 9, 12, 15, 18, 21,24, and 27° C.

Collecting data on individual fish represents an improvement in method.

The experiment also provides an opportunity for new students to learn and apply methods developed in earlier studies.

Photos by Charles Weibel

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KSU Aquaculture Delivers Koi to the Ark Encounter

Koi are a great option for aquaponic producers in Kentucky that are not interested in growing food fish but want still reap the benefits that aquaponics provides. Koi thrive at a wide temperature range (60-78F) making them a great fish candidate to grow year-round or even just during the colder months when heating a greenhouse for warm-water or tropical fish becomes cost prohibitive. After harvesting tilapia, the Ark Encounter wanted to restock their fish with Koi and reached out to the KSU aquaculture extension team. Having a plethora of Koi from the previous year’s spawning activities, Janelle Hager and Javier Ubilla, provided 75 fish for their system. The Ark encounter operates a geothermal greenhouse that contains 3000 sq ft of vertical NFT channels supported by four 300-gallon fish tanks. Lettuce grown in the system provides visitors with an opportunity to feed the animals on site. Visitors from all over the country tour the aquaponics greenhouse throughout the year, getting to see their aquaponics system in action.

Photos by Charles Weibel

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Second Street School Students Sample Fish

Approximately twelve 5th - 8th graders from Second Street School, Frankfort Ky, visited the Aquaculture Research Center. Students toured the aquaculture facility and a got to participated in a tilapia cooking demonstration. Fish is a good source of lean protein, omega-3 fatty acids, and various essential nutrients.

These students were apart of a program in cooperation with the Franklin Co Farmers Market that focused on local foods and healthy cooking. Many of the students tried tilapia for the fist time and enjoyed it.

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Dr. Semmens’s Fish Physiology and Morphology Class Examine Paddlefish

Kentucky State University graduate and undergraduate students traveled to the Harold R. Benson Research and Demonstration Farm to capture a few paddlefish for their Fish Physiology and Morphology Class 412/512.

Dawson and Noah set out gill nets and before the second net was set, they had fish in the nets. Paddlefish were removed from the gill nets and moved to the hauling tank on the truck and transported to the Aquaculture Research Center.

The pond at the Harold R. Benson Research and Demonstration Farm is used for aquaculture research, and holding brood stock of Paddlefish, Catfish and Largemouth bass.

Paddlefish, also known as spoonbill, are a unique species of fish that possess several distinctive characteristics:

Paddle-like rostrum: The most prominent feature of paddlefish is their elongated, paddle-shaped rostrum, or snout, which accounts for about one-third of their body length. This rostrum is flattened and extends forward like a spatula, giving them their common name.

Ancient lineage: Paddlefish belong to the family Polyodontidae and are often referred to as living fossils because they have existed for over 125 million years. They are considered one of the oldest surviving species of fish.

Filter-feeding mechanism: Paddlefish are filter feeders, using their specialized gill rakers to strain food particles from the water. They swim with their mouths open, allowing water to pass through their gill rakers while retaining plankton, small invertebrates, and fish larvae as their primary food source.

Lack of teeth: Unlike most other fish species, paddlefish lack true teeth in their mouths. Instead, they have roughened pads on the upper jaw and tongue, which aid in capturing and manipulating their prey.

Size and longevity: Paddlefish can grow to impressive sizes. The largest individuals can reach lengths of over 7 feet (2.1 meters) and weigh several hundred pounds. They are also known for their long lifespans, with some individuals living for more than 50 years.

Distribution: Paddlefish are native to North America and are primarily found in large rivers and reservoirs in the Mississippi River basin and the Missouri River basin. They were once more widespread, but their numbers have declined due to habitat loss and overfishing.

Caviar production: Paddlefish are valued for their roe, which is used to produce a type of caviar known as "paddlefish caviar" or "spoonbill caviar." The eggs are relatively small and have a unique flavor compared to other types of caviar.

Due to their distinctive physical features, ancient lineage, and unique feeding habits, paddlefish are considered an intriguing and ecologically important species. However, they are also facing conservation concerns and efforts are underway to protect and restore their populations.

Photos by Charles Weibel

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Team Ray Travels to Florida to visit the University of Miami

Dr. Andrew Ray, Mark Johannemann, and Sagun Chhetri traveled to Miami to visit with colleagues and tour the aquaculture facilities at the University of Miami (UM).

They visited with Dr. John Stieglitz, one of Dr. Ray's collaborators on a couple of grants, Dr. Stieglitz is heading up much of UM's finfish research. UM will provide olive flounder to the Ray lab later this year as part of that collaboration.

The team then traveled to Homegrown Shrimp Florida, an intensive commercial hatchery and shrimp farm in Indiantown, Florida, developed by Robins McIntosh, the Vice President of Charoen Pokphand Foods Public Company, the largest private company in Thailand. There, they visited with staff and toured the facilities as part of a grant, Dr. Ray has to survey the US shrimp farming industry.

Also, as part of that grant, Mark and Sagun traveled to Infini-SEA, a shrimp post larvae and broodstock-producing facility in Vero Beach, Florida. Infini-SEA is conducting exciting research into shrimp genetics and algae used to feed the larval shrimp.

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Noah Jenkins Stocks His Thesis Research Project

Noah Jenkins’s research project is a 12 week nutritional study featuring juvenile Largemouth bass. His study will be conducted in a recirculating aquaculture system, (RAS) that includes 28, 110 litter tanks, with 15 fish in each tank. He will be investigating seven diets including his control diet. Each diet has a replicate of four tanks. When the fish were stocked they weighed around three grams each.

Largemouth bass are popular among anglers for their sporting qualities and are often caught and released for recreational purposes. However, they can also be consumed as food.

The taste of largemouth bass can vary depending on the water it inhabits and its diet. Generally, the meat of largemouth bass is mild and has a slightly sweet flavor.

Many people enjoy the taste and texture of bass, finding it similar to other freshwater fish such as trout or walleye. The flesh is typically white, flaky, and low in fat. It can be prepared using various cooking methods, including grilling, baking, frying, or broiling.

Photos by Charles Weibel

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KSU Staff and students Visit Wolf Creek National Fish Hatchery

Tifani McKay had to deliver medicated feed to a Kentucky farmer last week. The farmer lived near Lake Cumberland. Therefore, Mrs. McKay took the opportunity to bring a few students to the Wolf Creek National Fish Hatchery, located below Wolf Creek Dam in Jamestown, Kentucky. They have an interactive Visitor Center and Environmental Education Center along with a Hatchery building and raceways where fish production can be observed.

An estimated 1,000,000 trout weighing 275,000 pounds are produced each year. These fish provide over $50 million dollars per year in direct economic benefits. These fish are stocked in coordination with the Kentucky Department of Fish and Wildlife Resources in over 125 public fishing waters within the commonwealth of Kentucky.

Students had the chance to observe a few trout species as well as seeing large indoor and outdoor raceways in use.

Photos by Charles Weibel

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Jill Fisk Demonstrates (TSS/VSS)

 

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Team Semmens Launch Floating Raceway in London

James Ulrich and his brother Dennis (London KY) have been collaborating with KSU on the floating raceway project for several years. Their pond is stocked with catfish. There are no bluegill or bass so the catfish can freely reproduce. They trap catfish from the pond and grow them to food size.

Over a two-day period, a second smaller raceway was recently constructed on-site by a crew of four and launched into the pond. It will provide flexibility to trap and grow small catfish from the pond as stockers to put in the larger raceway for grow-out. Installation of the air blower, grid airlift, and screens will require another trip.

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USDA AgDiscovery Camp Visit the Aquaculture Research Center

On June 13, 2023, approximately 25 high school students from the USDA AgDiscovery Camp stopped into the Kentucky State University Aquaculture Research Center (ARC). During their visit; they were shown active aquaculture research projects, talked to researchers and participated in a fish anatomy lesson. AgDiscovery is a free summer outreach program that helps teenagers explore careers in agricultural sciences. The students stay on campus’ for 2–4 weeks and learn from university professors and USDA experts.

This year, 22 colleges and universities are participating in AgDiscovery. Each school’s program focuses on a specific area of interest to our Nation’s agriculture; from plant health, entomology, and biotechnology to veterinary science, animal welfare, food science, and agribusiness.

The students that applied for the Animal Science discipline of the camp will travel to Alcorn State University, Florida A&M University, Fort Valley State University, Virginia State University and Kentucky State University. This is a unique opportunity for students to explore agriculture career paths and to build lasting leadership skills.

Photos by Charles Weibel

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SWAT Training

The Soil and Water Assessment Tool (SWAT) is a small watershed-to-river basin- scale model used to simulate the quality and quantity of surface and groundwater and predict the environmental impact of land use, land management practices, and climate change.

SWAT is widely used in assessing soil erosion prevention and control, non-point source pollution control, and regional management in watersheds.

This 4-day training is for graduate students and early career experts in forestry, agronomy, hydrology, and related fields. Background in hydrology, soils, ecology, and GIS/Remote sensing is advisable.

The Aquaculture Research Center sent graduate students Ashton Schardt, Uchechukwu Ohajundu, and Guyana Rana to the training.

Photo by Charles Weibel

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Team Semmens Christen Another Floating Raceway

Team Semmens’s launched the most recent version of a 9.5 cubic meter suspended floating raceway in the two-acre pond at the KSU research farm. Wheels were placed under the raceway and it was backed into the pond with a skid steer like launching a 32-foot boat. An inverted International Bulk Container, (IBC), was installed for floatation as the first end entered the water. After it was maneuvered into deeper water a second tote IBC float was secured and the unit was floated into position. The wheels were removed after the raceway was floated into position.
This is the third launch for this size raceway at the KSU research farm pond. It will be stocked with Largemouth bass, (LMB), fry. LMB fry in this first phase rely on natural food (zooplankton) pumped into the raceway for the first 40 days of life. They will be harvested later, and trained to feed on a pelleted diet before stocking in phase two for the rest of the year. The raceway system will be reconfigured with a grid airlift to increase water flow during the second phase.

Photos by Charles Weibel

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Femi Completes His Thesis Research Project

Oluwafemi Adebayo,is graduate student on the Aquaculture Nutrition Team, and is under the supervision of Dr. Rossi. Mr. Adebayo, AKA Femi, completed his thesis research last week. His growth trial concentrated on the efficacy of modified black soldier fly larvae in diets of pacific white shrimp. Black soldier fly meal has been recognized as a feed ingredient in aqua feeds because it is rich in protein like fishmeal.

The shrimp were weighed by batch/tank to measure growth. The whole body was collected for proximate analysis and their hemolymph and intestines were also extracted for consequent analysis.

Photos by Charles Weibel

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United States Shrimp Farming Survey

Dr. Ray, in partnership with Dr. Mara Merlino from the KSU School of Behavioral and Social Sciences, has created a survey designed to assess the status of the United States Shrimp Farming Industry. The two researchers received a grant from the USDA Animal and Plant Health Inspection Service to fund this survey work. The survey covers topics such as the types of production systems and rearing practices used, broodstock and grow-out management, nutrition, health monitoring and biosecurity, marketing, and identification of bottlenecks and needs from government or academic institutions. The team will use this information to create a detailed report on the industry that will help guide research, regulatory, or incentive-based programs to help the industry grow. So far over 150 active shrimp farms have been identified and invited to participate. If you know of any shrimp farmers, fresh water or salt water, please pass along the survey invitation to them.

Click Here for Survey

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Mark Johanemann Successfully Defends Thesis

Mr. Johannemann presented his thesis research to an audience of around 35 people at the Aquaculture Research Center on May 1, 2023. He then defended his research before his Committee, Dr. Ken Semmens, Dr. Noel Novelo, and his Major Professor Dr. Andrew Ray. The title of Mark’s research is “THE EFFECTS OF CARBON SOURCE AND WOODCHIP MEDIA IN DENITRIFICATION FILTERS ON WATER QUALITY AND SHRIMP PRODUCTION IN HYBRID RAS.”

Mark was an undergraduate who worked at the Aquaculture Research Center, he was the treasurer of the WAS Aquaculture Sub-unit and is the current president of the Sub-unit.

Photo by Charles Weibel

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KSU subunit of the United States Aquaculture Society Tours the Louisville Zoo

On Thursday, April 18, 2023, the KSU subunit of the United States Aquaculture Society visited the Louisville Zoo for a tour and presentation of the aquatic life support systems. This presentation and tour were given by Aquatic Life Support Technician and Jodie Bennet and Engineer Lonnie Lamb. During the visit subunit members learned about the history of the life support systems at the Louisville Zoo as well as the process of designing and operating systems to meet the specific needs of the individual animals. After the tour members were able to tour the rest of the zoo and view other systems.

Photos by Charles Weibel

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Oluwafemi, “Femi” Has Started His Research in the Nutrition Lab

Oluwafemi Adebayo is conducting a growth trial that will evaluate a modified black soldier fly meal in pacific white shrimp, Litopenaeus Vannamei. This research is a collaboration between Texas A&M University and Kentucky State University.

The increasing high cost of fish meal as an animal protein source in the diet of aquatic animals has necessitated the need for alternative protein source that will be cheaper and nutritionally viable for aquatic species. Black soldier fly meal has been found to be rich in nutrients such as protein and high-quality amino acids that can be used in diets of animals as an alternative protein source. This experiment is designed with 7 diets (5 replicates) and with increasing black soldier fly inclusion levels and a control treatment to be fed with commercial feed.

Twelve shrimp (1g/shrimp) are stocked in 35, 110L-glass aquaria. The study will last between 8 to 12 weeks. At the end of the research trial growth parameters will be evaluated.

Photos by Charles Weibel

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Aquatic Farming Newsletter Spring 2023

Click Here For Download

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Mark Johannemann Featured in the Fall 2022 Edition Newsletter of the Fish Culture Section of the American Fisheries Society

Click Here To Download Full Newsletter

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Gyanu Rana Completes Her Thesis Research Project

The topic of Gyanu’s research was “The Effects of System Types and Horizontal Substrate Addition on Pacific White Shrimp (Litopenaeus vannamei) Production and Water Quality in RAS”. Her study objective was to compare Clearwater System with Hybrid-water System and assess the effects of horizontal substrate addition on Pacific White Shrimp production.

This experiment had four treatments: Clear- water with Substrate, Clear-water with no substrate, Hybrid-water with the substrate, and Hybrid-water with no substrate. Each treatment had four replicates stocked at the rate of 450 shrimp/m3.

This experiment ran for 53 days. Her shrimp were harvested and had an average weight of 17 grams. The Clear-Water with Substrate treatment had the highest survival rate of nearly 90%

Photos by Charles Weibel

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Research on the Microbiome of Aquaponic Systems Aims to Improve Sustainability Through Nutrient Recovery

Janelle Hager and James Ward are conducting research on the microbiome of plants grown in aquaponic systems. Over the next four months, they will collect water and root samples from bell pepper plants at five different life stages – plantlet, vegetative, first bud/flower, fruit set, and fruit ripening. The DNA and RNA from the microbial communities will be extracted and analyzed for species diversification and regulation of the phosphatase enzyme. Extracellular phosphatase will also be monitored on the roots and in the water. Understanding the microbial communities and how they impact phosphorous (P) availability at different life stages will help improve nutrient management of aquaponics systems.

Phosphatase is a key enzyme in converting P from an organic to inorganic form that is available to the plants. In soil, plants recruit bacteria to their rhizosphere (area around the roots) that make nutrients in the soil available to the plants. These mechanisms are not understood in aquaponic systems. Bacteria are the engine behind aquaponic systems; however, very little is known about their composition or function.

So why not just add more P for our plants? Global rock phosphate reserves are projected to be depleted within 50 years. Nutrients trapped in the solid effluent of fish waste can comprise up to 60% of the P from feed. Figuring out how to fully use the nutrients already in the system is key to improving the sustainability of aquaponics systems.

Funds for this research are provided by USDA AFRI-Agricultural Microbiomes.

Can fish waste help improve soil quality for hay production? One KY farmer thinks so.
While waiting for a friend to have cataract surgery, I got to talking with a man who was accompanying his wife for the same surgery. As we chatted, we got on the subject of mineralization work I was doing at KSU. Mineralization is the microbial conversion of organic nutrients to inorganic nutrients – think composting for solid fish waste. In aquaponics, we use nutrient rich water from fish production to grow hydroponic plants. However, the solid portion of the waste is removed leaving only dissolve nutrients in the water. The fish effluent is moved to a large IBC tote and aerated for 10 days. The solids are allowed to settle and the nutrients in the water are put back in the system for plant growth. We have seen between 75-175% increase in nutrients such as P, N, Ca, K, and Mg through the mineralization process.

Stephen Gibson and his wife, Zina, were interested in using it as a fertilizer on their hay fields. Currently, they use 36-0-0 (NPK), 19-19-19, and lime for their fields. The current rate of Lime application for their fields is 3-ton/acre. At 25 acres of hay production, that it a lot of lime – and money. So I loaded up 250 gallons of mineralized fish effluent and dropped it at their farm in Owsley Co. Stephen is planning to test application rates and sprayer nozzles on different areas on his fields this growing season. In addition, he wants to look at using the intact slurry, the clear water without solids, and diluted concentrated solids.

To our knowledge, the Gibson’s are the only folks in Kentucky doing field application on fish effluent on land used for hay production. To put it in perspective, a gallon of liquid fish emulsion at Lowes will run you $18. Put’s a whole new spin on liquid gold!

Photos by Charles Weibel

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October 2022 Aquatic Farming News

Click Here To Download Newsletter

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Dan Moreland Freshwater Prawn Harvest

 

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Dr. Ken Thompson Publishes Article in World Aquaculture Magazine

Click Here To Download Full Article

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Mark Johannemann's Recirculating Aquaculture System

 

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Jasmine Iniguez Highlights The Cover of the American Fisheries Society Newsletter

Click Here To Download Full Newsletter

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Ray Lab Publishes Magazine Article

An article was recently published in the Responsible Seafood Advocate Magazine which summarized some of the work that the Ray Lab has done regarding the creation and evaluation of a home-made sea salt mixture for shrimp production. Artificial sea salt can be a major expense for inland marine shrimp farmers. The Ray Lab just completed a four-year project funded by the USDA AFRI Program looking at making inexpensive sea salts. The project was led by Dr. Ray's Lab and included collaborators at Auburn and Purdue Universities. Ultimately, the team was very successful in demonstrating that a home-made sea salt cost half that of commercial salts, and produced equivalent results. Several farmers in Kentucky and across the region have already adopted this new formulation.

Click Here For Full Article

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Dr. Andrew Ray’s lab has published a new article in the peer-reviewed journal Aquaculture Reports. A previous study from the Ray lab found highly varied saltwater shrimp performance when using a low-cost, homemade salt mix (LCS), while a commercial sea salt mix (CSS) and 3 different ratios of both mixes (75%:25%, 50%:50%, 25%:75%) showed more consistent production. This new study more closely examined 100% use of the LCS mixture in shrimp production, along with 5 other ratios of LCS:CSS from 97.5%:2.5% up to 75%:25%. This study found no difference in shrimp production using increased amounts of the LCS mixture, low variability across all treatments, high shrimp survival and growth rates, and decreased production costs when using the LCS mixture. This study has important implications for shrimp producers, as salt costs represent a significant portion of the reoccurring costs in shrimp farming and use of the LCS mixture reduces salt cost by 65% when compared to a CSS mixture. There are already several shrimp farmers both in Kentucky and abroad who have adapted this mixture and reported decreased production costs and no changes in shrimp performance.

Click Here to Download Full Journal Article

 

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James Brown Featured in the Student Spotlight of the American Fisheries Society Fish Culture Section Newsletter

Click Here For Full Newsletter

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Kentucky State University's Aquaculture Teacher's Manual

Click Here For Full Manual

 

 

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Floating Raceway Design For Small Farms

Dr. Semmens crew is developing a floating raceway design for small farms and have recently scaled up a suspended design that can be constructed at modest cost on the small farm. It was stocked with 5000 feed trained largemouth bass fingerlings produced by students and staff at the Aquaculture Research Center to obtain preliminary data. We are growing catfish in our other raceways, but bass represent a higher value fish that has not been evaluated in this format. We expect there will be advantages with feeding, and management of the bass, but cannibalism and rapid fish growth are a concern.

The bass were stocked into a raceway with 9 cubic meters of growing space. The raceway frame is 32 feet long and the raceway itself is about Four feet deep and 20’ long with about eight inches of freeboard. The bass stocked in the raceway will be grown out as food fish in other raceways next year. We will assess performance of the bass in the raceway this fall.

Photos by Charles Weibel

 

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Kentucky State University's Aquaponics Production Manual: A Practical Handbook For Growers

The School of Aquaculture and Aquatics Sciences at Kentucky State University is proud to announce the availability of the Aquaponics Production Manual: A Practical Handbook for Growers. This 75 page comprehensive manual covers the biological concepts of aquaponics, types of systems, suitable fish and plant species, systems management, water quality, disease of plants and fish, controlled environments (greenhouse and indoors), marketing and economics, as well information on certification and regulations. It is written at a level to be practical resource for practicing (or potential) aquaponic producers. 

Authors: JANELLE HAGER, LEIGH ANNE BRIGHT, JOSH DUSCI, & JAMES TIDWELL

CLICK HERE FOR COMPLETE MANUAL

 


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EVENTS

 

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Aquaculture Online Courses

Blue ButtonFisheries for the Educated Consumer
This Internet class features video lectures over PowerPoint slides. Instructor: Dr. Ken Semmens

Pink Button Water Quality Management
Water chemistry as it relates to aquaculture and recreational pond management.

robert.durborow@kysu.edu
SEE VIDEO CLIP

Green ButtonAquaponics
This course will provide an overview of principles and practices of aquaponic production. Instructor: Dr. James Tidwell and Janelle Hager

Brown button Fish Reproduction & Spawning Techniques
This course includes recorded lectures and practical demonstration videos. Instructor: Dr. Boris Gomelsky
boris.gomelsky@kysu.edu
SEE VIDEO CLIP

Green ButtonFish Genetics
Emphasis is on practical applications in Aquaculture and Fisheries. Instructor: Dr. Boris Gomelsky boris.gomelsky@kysu.edu
SEE VIDEO CLIP

Blue ButtonPrinciples of Aquaculture
This Internet class features video lectures over PowerPoint slides. Instructor: Dr. Jim Tidwell james.tidwell@kysu.edu
SEE VIDEO CLIP



red button Fish Diseases
This course taught by Dr. Robert Durborow
robert.durborow@kysu.edu
SEE VIDEO CLIP

 

Yellow Button Survey of Production Methods
This Internet class features video lectures over PowerPoint slides. Instructor: Dr. Jim Tidwell james.tidwell@kysu.edu

 

 
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